Molecular Weight : 1129.85      
Molecular Formula : C₅₁H₅0Cl₂N₂O₂₃           
Purity ≥ 98%(HPLC)
Ex(nm) : 506nm         Em(nm) : 526nm

Introduction
Since being introduced in 1989, fluo-3 imaging has revealed the spatial dynamics of many elementary processes in Ca²⁺ signaling. Fluo-3 has also been extensively used for flow cytometry; for experiments involving photoactivation of “caged” chelators, second messengers, and neurotransmitters;and for cell-based pharmacological screening.The most important properties of fluo-3 in these applications are an absorption spectrum compatible with excitation at 488 nm by argon-ion laser sources, and a very large fluorescence intensity increase in response to Ca²⁺ binding.
After receipt, these products should be stored desiccated and protected from light at ≤ -20°C; AM esters are susceptible to hydrolysis (particularly in solution) but can be stored at least six months in the vials as received. The AM esters should be reconstituted in anhydrous DMSO then used as soon as possible thereafter (within a week) to avoid decomposition with subsequent loss of cell loading capacity. DMSO stock solutions of AM esters should be stored protected from light, frozen,and desiccated. Stock solutions of the salts may be prepared in distilled water or aqueous buffers and stored frozen (≤ -20°C) and protected from light; these solutions should be stable for at least six months.

Storage condition
-20°C and protect from light

For Research Use Only. Not For Use In Diagnostic Procedures.