Chaps Cell Extract Buffer (10X)

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产品货号

产品名称

CAS号

包装

市场价（人民币）

库存状态

MCC2967

Chaps Cell Extract Buffer (10X)

10ml

1280.00

In stock

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产品概述
Product Description Chaps Cell Extract Buffer can be used to lyse cells under nondenaturing conditions and is recommended for the preparation of cytoplasmic cell lysates. Product Usage Information Treat cells by adding fresh media containing regulator for desired time. Aspirate media from cultures; wash cells three times with PBS; aspirate. Scrape cells into PBS and spin down to pellet. Lyse cells by adding Chaps Cell Extract Buffer (1 volume of cell pellet). Resuspend cells in the buffer, freeze and thaw three times, centrifuge lysate at 14,000 rpm. Keep the supernatant and discard the pelleted cell debris. Add SDS Sample Buffer and heat sample to 95–100°C for 5 minutes; cool on ice. Microcentrifuge for 5 minutes. Load 5 µL onto SDS-PAGE gel (10 cm x 10 cm). Solutions and Reagents CHAPS Cell Extract Buffer (5mL) 1X concentration: 50 mM Pipes/HCl (pH 6.5), 2 mM EDTA, 0.1% Chaps, 20 µg/ml Leupeptin, 10 µg/ml Pepstatin A,10 µg/ml Aprotinin. Add DTT to 5mM.1.25 M (250X) DTT (0.25 mL). Storage condition Supplied as a 10X solution. Store at -20°C For Research Use Only

产品概述

Product Description

Chaps Cell Extract Buffer can be used to lyse cells under nondenaturing conditions and is recommended for the preparation of cytoplasmic cell lysates.

Product Usage Information

Treat cells by adding fresh media containing regulator for desired time. Aspirate media from cultures; wash cells three times with PBS; aspirate. Scrape cells into PBS and spin down to pellet. Lyse cells by adding Chaps Cell Extract Buffer (1 volume of cell pellet). Resuspend cells in the buffer, freeze and thaw three times, centrifuge lysate at 14,000 rpm. Keep the supernatant and discard the pelleted cell debris. Add SDS Sample Buffer and heat sample to 95–100°C for 5 minutes; cool on ice. Microcentrifuge for 5 minutes. Load 5 µL onto SDS-PAGE gel (10 cm x 10 cm).

Solutions and Reagents

CHAPS Cell Extract Buffer (5mL) 1X concentration: 50 mM Pipes/HCl (pH 6.5), 2 mM EDTA, 0.1% Chaps, 20 µg/ml Leupeptin, 10 µg/ml Pepstatin A,10 µg/ml Aprotinin. Add DTT to 5mM.1.25 M (250X) DTT (0.25 mL).

Storage condition

Supplied as a 10X solution. Store at -20°C

For Research Use Only

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