Description
The MesGenTM Endo-free Plasmid Miniprep Kit is based upon a modified SDS-alkaline lysis of bacterial cells in combination with selective binding of the plasmid DNA to a column. Each column has a binding capacity of at least 20 μg （Endotoxin <0.1EU/μg plasmid DNA）, The protocol provides a simple and reliable method to achieve the rapid isolation of highly purified plasmid DNA. The kit can also be used to isolate large recombinant constructs, such as BACs, PACs and P1s but some decrease in elution efficiency may be encountered. The protocol has been optimized for bacterial cultures grown in LB (Luria-Bertani) broth, but can also be used for cultures grown in rich broths, such as LBG (LB + 2% glycerol) and 2 × YT. TB (Terrific Broth) is not recommended for use with this kit. Each column can process up to 4 ml of bacterial culture grown in LB or up to 2 ml of culture grown in rich broth. The entire procedure can be completed within 1 hour. The highly purified plasmid DNA is eluted in a small volume of Tris buffer eluent or deionized water and can be used immediately for many routine applications, such as DNA sequencing, restriction digestion, in vitro transcription, library screening, ligation and transformation.

Storage condition 
RNase A : Store at room temperature. If a precipitate is present, use an aliquot of Soultion I to resuspend and transfer to the Soultion I bottle.
Soultion I : Resuspension buffer. Store at 4°C after addition of RNase A.
Soultion II  : Lysis buffer. Store at room temperature.
Soultion III  : Neutralization buffer. Store at 4°C.
Endo-Out Solution : Store at 4°C
Solution PE : Store at room temperature.
DNA Wash Buffer : Store at room temperature.
Elution Buffer : Store at room temperature. 

For Research Use Only. Not for use in diagnostic procedures.