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Description
IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening. Ready-to-use 100mM IPTG aqueous Solution is non-toxic, stable, 0.22µm membrane-filtered, formulated for direct use in blue/white colony screening.
Applications
1 : Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies.
2 : Induction of gene expression under control of the lac promoter.
Note
1 : For blue/white colony screening use 0.1 mM final IPTG concentration in LB (Luria Broth) media.
2 : Store IPTG Solution at -20°C.
For Research Use Only. Not For Use In Diagnostic Procedures.
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