Laemmli Sample Buffer (odor-free), 2X

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MG0105

Laemmli Sample Buffer (odor-free), 2X

-	10ml	150.00	In stock
-	25ml	280.00	In stock

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产品概述
Description Laemmli Sample Buffer (odor-free), 2X is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The DTT reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well. Solutions and Reagents 2X solution contains 4% SDS, 20% glycerol, 10% 400 mM DTT, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. Storage condition -20° C For Research Use Only

产品概述

Description

Laemmli Sample Buffer (odor-free), 2X is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The DTT reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.

Solutions and Reagents
2X solution contains 4% SDS, 20% glycerol, 10% 400 mM DTT, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8.

Storage condition
-20° C

For Research Use Only

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