Mes-Red葡萄糖/葡萄糖氧化酶检测试剂盒 Mes-Red Glucose/Glucose Oxidase Assay Kit

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MGG3839

Mes-Red葡萄糖/葡萄糖氧化酶检测试剂盒 Mes-Red Glucose/Glucose Oxidase Assay Kit

500 tests

2450.00

In stock

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产品概述
Introduction The Mes-Red Glucose/Glucose Oxidase Assay Kit provides a sensitive one-step method for detecting glucose or glucose oxidase. The Mes-Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) is a colorless, stable, and extremely versatile peroxidase substrate.Because peroxidase- and glucose oxidase–mediated reactions can be coupled, it is possible to measure glucose oxidase activity or the release of glucose by any glucosidase enzyme—for instance, β-glucosidase and glucocerebrosidase—in either a continuous or discontinuous assay.This assay should also be very useful for quantitation of glucose levels in foods, fermentation media, and bodily fluids. In the assay, glucose oxidase reacts with d-glucose to form d-gluconolactone and H2O2. In the presence of horseradish peroxidase (HRP), the H2O2 then reacts with the Mes-Red reagent in a 1:1 stoichiometry to generate the red-fluorescent oxidation product, resorufin.Resorufin has fluorescence excitation and emission maxima of approximately 571 nm and 585 nm, respectively, and because the extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically. Furthermore, at these long wavelengths, there is little interference from autofluorescence found in most biological samples. With the Mes-Red Glucose/Glucose Oxidase Assay Kit, we have detected as little as 3 μM d-glucose and 0.05 mU/mL glucose oxidase. Allow reagents to warm to room temperature before opening vials. The Mes-Red reagent is somewhat air sensitive. Once a vial of Mes-Red reagent is opened, the reagent should be used promptly. Storage conditions The kit is shipped on ice. Store all components at -20°C. Shelf life: 6 months after receipt. For Research Use Only. Not For Use In Diagnostic Procedures.

产品概述

Introduction

The Mes-Red Glucose/Glucose Oxidase Assay Kit provides a sensitive one-step method for detecting glucose or glucose oxidase. The Mes-Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) is a colorless, stable, and extremely versatile peroxidase substrate.Because peroxidase- and glucose oxidase–mediated reactions can be coupled, it is possible to measure glucose oxidase activity or the release of glucose by any glucosidase enzyme—for instance, β-glucosidase and glucocerebrosidase—in either a continuous or discontinuous assay.This assay should also be very useful for quantitation of glucose levels in foods, fermentation media, and bodily fluids.

In the assay, glucose oxidase reacts with d-glucose to form d-gluconolactone and H2O2. In the presence of horseradish peroxidase (HRP), the H2O2 then reacts with the Mes-Red reagent in a 1:1 stoichiometry to generate the red-fluorescent oxidation product, resorufin.Resorufin has fluorescence excitation and emission maxima of approximately 571 nm and 585 nm, respectively, and because the extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically. Furthermore, at these long wavelengths, there is little interference from autofluorescence found in most biological samples. With the Mes-Red Glucose/Glucose Oxidase Assay Kit, we have detected as little as 3 μM d-glucose and 0.05 mU/mL glucose oxidase.

Allow reagents to warm to room temperature before opening vials. The Mes-Red reagent is somewhat air sensitive. Once a vial of Mes-Red reagent is opened, the reagent should be used promptly.

Storage conditions

The kit is shipped on ice. Store all components at -20°C. Shelf life: 6 months after receipt.

For Research Use Only. Not For Use In Diagnostic Procedures.

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