Background
Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration (PMID:19479947). It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases (PMID:12549038). It can exsit as a dimer (PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

Immunogen
Citrate synthase fusion protein Ag9117

Source
Rabbit

Isotype
IgG

Purification method
Antigen affinity purification

Species Specificity
human, mouse, rat

Calculated molecular weight
466 aa, 52 kDa

Observed molecular weight
45-50 kDa

Uniprot
O75390

Tested Applications
IF, IHC, IP, WB,ELISA

Recommended dilution
WB : 1:1000-1:8000
IHC : 1:100-1:400
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IF : 1:50-1:500

Storage condition
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

For Research Use Only. Not For Use In Diagnostic Procedures.